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当前位置：化学与化工研究 > 2024年 4卷 (2)期

Open Access Article

Journal of Chemistry and Chemical Research. 2024; 4: (2) ; 12-20 ; DOI: 10.12208/j.jccr.20240011.

Sodium hyaluronate full spectrum skincare efficacy testing
全谱透明质酸钠护肤功效测试

作者： 王朋田¹ *, 刘宝珍¹, 朱金会², 张敏¹, 梁亚妮¹

1 山东众山生物科技有限公司山东日照

2 山东众山生物科技有限公司青岛分公司山东青岛

*通讯作者：王朋田,单位：山东众山生物科技有限公司山东日照；

发布时间: 2024-12-31 总浏览量: 73

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摘要

鉴于传统透明质酸钠（HA）的生产主要集中在单一分子量分布的产品，分子量分布范围狭窄，无法满足皮肤从表皮到真皮层的全方位保湿与修护需求，本研究通过调整生产工序制备分子量分布更广的全谱透明质酸钠（全谱HA）。采用体外、体内测试方法对全谱HA的护肤功效进行测试，并测试其使用安全性。采用3D-人体表皮皮肤模型，在体外定量检测不同分子量HA的渗透性，结果显示，全谱HA具有良好的经皮渗透性，24h皮下扩散百分率达到12.9%。采用人原代真皮成纤维细胞模型，利用酶联免疫吸附法定量检测全谱HA对弹性蛋白的上调率，结果显示，全谱HA在0.1%-1.0%（m/v）范围内能显著提升弹性蛋白的合成。人体面部皮肤测试结果显示，使用含有0.5%（m/v）全谱HA面霜的0.5 h、2 h、4 h、28天后，具有一定的保湿修护、紧致抗皱的功效。人体皮肤斑贴测试结果显示，0.5%浓度（m/v）的全谱HA未导致皮肤出现不良反应，具有良好的使用安全性，为其在日用化妆品领域的应用提供有力数据支持。

关键词： 化妆品；全谱透明质酸钠；经皮渗透性；弹性蛋白；保湿；修护；紧致；抗皱

Abstract

Given that the production of traditional sodium hyaluronate (HA) mainly focuses on products with a single molecular weight distribution, the molecular weight distribution range is narrow and cannot meet the comprehensive moisturizing and repairing needs of the skin from the epidermis to the dermis layer. This study adjusted the production process to prepare a wider molecular weight distribution of sodium hyaluronate full spectrum. Use in vitro and in vivo testing methods to test the skincare efficacy of sodium hyaluronate full spectrum and assess its safety. Using a 3D human epidermal skin model, the permeability of HA with different molecular weights was quantitatively detected in vitro. The results showed that sodium hyaluronate full spectrum had good transdermal permeability, with a 24-hour subcutaneous diffusion rate of 12.9%. Using a human primary dermal fibroblast model, the upregulation rate of elastin by sodium hyaluronate full spectrum was quantitatively detected using enzyme-linked immunosorbent assay. The results showed that sodium hyaluronate full spectrum could significantly enhance elastin synthesis within the range of 0.1% -1.0% (m/v). The test results of human facial skin show that 0.5 h, 2 h, 4 h and 28 days after the use of 0.5% (m/v) sodium hyaluronate full spectrum face cream, it has certain moisturizing, repairing, tightening and anti wrinkle effects. The results of human skin patch testing showed that the sodium hyaluronate full spectrum at a concentration of 0.5% (m/v) did not cause adverse reactions on the skin and had good safety, providing strong data support for its application in the field of daily cosmetics.

Key words： Cosmetics; Sodium hyaluronate full spectrum; Transdermal permeability; Elastin; Moisturizing; Repair; Firming; Anti-wrinkle

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引用本文

王朋田, 刘宝珍, 朱金会, 张敏, 梁亚妮, 全谱透明质酸钠护肤功效测试[J]. 化学与化工研究, 2024; 4: (2) : 12-20.

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